Part:BBa_K2349002:Design

SUC2

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 787
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1510
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1088
  Illegal SapI.rc site found at 415
  

Design Notes

Addition of prefix and suffix and removal of illegal restriction site in the codon of amino acid 277 from T to G

Source

Amplified from genomic DNA of S. cerevisiae CENPK 21C with primers GAATTCGCGGCCGCTTCTAGATGCTTTTGCAAGCTTTCCTTTTCC and CTGCAGCGGCCGCTACTAGTACTATTTTACTTCCCTTACTTGGAACTTGTCAATG